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CoLocalizer Pro Changelog

What's new in CoLocalizer Pro 7.0

Aug 4, 2020
  • Introducing ML Super Resolution. Revolutionary Machine Learning-powered transformation of conventional fluorescence images to the images comparable with super resolution.
  • Now requires macOS 10.13 Hight Sierra or newer.

New in CoLocalizer Pro 6.1 (May 5, 2020)

  • Prediction accuracy of the Background Correction model has improved to 99%.
  • When changing channel pairs with Reveal Colocalized Pixels on, pixels on the image were not updated. Fixed.
  • It is now possible to export either uncompressed and compressed (LZW, Packbits) TIFF files.
  • Improved layout of the report files.

New in CoLocalizer Pro 6.0 (Dec 23, 2019)

  • Introducing Machine Learning-enhanced background correction. Deep Learning model provides over 90% prediction accuracy of various background noise levels.
  • Redesigned Background Correction pane, updated tooltips.
  • Histogram channels are now shown more distinctively.
  • Updated to the latest version of Sparkle update framework.
  • Updated graphics.

New in CoLocalizer Pro 5.6 (Oct 25, 2019)

  • Compatible with macOS 10.15 Catalina, including support for Sidecar.
  • Completely reworked functionality of Slice Navigator.
  • Added the Show Original button to the Background Correction pane.
  • Added support for Undo/Redo when changing channel pairs.
  • Improved layout of colocalization report.
  • Made various UI and performance enhancements.
  • Now requires OS X 10.11 El Capitan or newer.

New in CoLocalizer Pro 5.5 (Jul 25, 2019)

  • Complete UI overhaul: Background Correction panel and Colocalization sheet are now incorporated into the main window sidebar to streamline workflow and provide a modern single-window experience.
  • Made numerous related UI changes.
  • Added support for zoom and scroll gestures on a trackpad.
  • Completely reworked and simplified zoom resulted in combining five separate buttons into one.
  • Upped maximum zoom to 10000.
  • Inspector now shows the number of slices in stacks images.
  • Manipulations with slices in Slice Navigator, such as deleting and selecting, now work more reliably.
  • Better undo-redo when selecting ROI, rotating, and flipping.
  • Simplified results sharing options.
  • Reorganized and simplified colocalization report.

New in CoLocalizer Pro 5.4.6 (Jun 17, 2019)

  • Rearranged default toolbar.
  • Made various UI and performance enhancements.

New in CoLocalizer Pro 5.4.5 (Jun 11, 2019)

  • Updated links in Help.

New in CoLocalizer Pro 5.4.4 (Jun 7, 2019)

  • Updated License Agreement.
  • Made various UI and performance enhancements.

New in CoLocalizer Pro 5.4.3 (Jan 8, 2019)

  • Improved performance.

New in CoLocalizer Pro 5.4.2 (Nov 8, 2018)

  • Further refinements to Light and Dark Modes on macOS 10.14.
  • Made performance enhancements.

New in CoLocalizer Pro 5.4.1 (Oct 17, 2018)

  • Fixed an issue when settings for toolbar were not autosaved.

New in CoLocalizer Pro 5.4 (Oct 17, 2018)

  • Added support for Dark Mode.
  • Redesigned toolbar icons and several UI elements.
  • Made various performance enhancements.

New in CoLocalizer Pro 5.3.4 Build 454 (Jul 19, 2018)

  • Reduced the number of scattergram in image scale options when exporting data from 5 to 3.
  • Reduced the number of scattergram in image placement options when exporting data from 4 to 2.
  • Updated toolbar tooltips.

New in CoLocalizer Pro 5.3.3 Build 451 (May 17, 2018)

  • Renamed presets for background correction to Low Noise, Average Noise, and High Noise for better understanding of their use.

New in CoLocalizer Pro 5.3.2 Build 449 (Apr 30, 2018)

  • Zero histogram values of channels are not displayed now.
  • Improved histogram appearance.
  • Made various improvements of performance.

New in CoLocalizer Pro 5.3.1 Build 445 (Apr 20, 2018)

  • Improved wording of alerts.

New in CoLocalizer Pro 5.3 Build 443 (Apr 18, 2018)

  • Made more reliable algorithm for revealing colocalized pixels.
  • Scattergram is now presented as heatmap.
  • When revealing colocalized pixels, scattergram now shows the area where colocalized pixels are located.
  • When quantifying colocalization, image is now shown according to the pair of selected channels instead of three channels as it was before.
  • Fixed an issue when label displaying size of selected ROI could go beyond the main window when resizing it.
  • About Box now shows expiration date of the license.
  • Reorganized contents of default toolbar.
  • Reorganized Help menu items.
  • Made minor UI updates.

New in CoLocalizer Pro 5.2.3 Build 435 (Mar 2, 2018)

  • When merging stacks images, slices are now preserved.
  • When splitting stacks images, slices are now preserved.
  • Fixed an issue when newly merged files could not be analyzed without first saving them.

New in CoLocalizer Pro 5.2.2 Build 431 (Jan 5, 2018)

  • Added and updated various tool-tips.
  • Added keyboard shortcut for exporting images in different file formats: Command-E.
  • Made Background Correction panel more compact.

New in CoLocalizer Pro 5.2.1 Build 425 (Nov 22, 2017)

  • Updated several tooltips of toolbar icons and items in Colocalization window.
  • Fixed an issue when glossary pages in Help were opening in browser instead of in Help viewer on macOS 10.13 High Sierra.
  • Made minor UI tweaks.

New in CoLocalizer Pro 5.2 Build 416 (Sep 23, 2017)

  • Introducing Smart Background Correction: it is now possible to correct background regardless of the properties of Selected ROI and properly compare results from different experiments.
  • Enabled export of files in .colocalizer format: File > Export As... > CoLocalizer.
  • Changed the way how applied background correction settings are displayed in the Inspector.
  • Changed the way how file path is displayed in the Inspector.
  • Fixed an issue when title in the Background Correction panel of stacks images was not updated to indicate the number of the moved slice.
  • Fixed an issue when .colocalizer file was not created properly when user wasn't signed in to iCloud account.
  • Fixed minor compatibility issues on OS X 10.10 and 10.11.
  • Now requires OS X 10.10 (Yosemite) or newer.

New in CoLocalizer Pro 5.1 Build 403 (Jul 8, 2017)

  • Redesigned Background Correction panel.
  • Removed the option to correct background using background ROI.
  • Background correction and histogram display are now performed using Core Image Filter to achieve significantly higher performance.
  • Made more intelligent zoom, max zoom is upped to 1000% to be on par with iOS version.
  • Rotation and flipping are now synced between devices.
  • Improved appearance of thumbnails in slice navigator.
  • Added Yosemite-like vibrancy appearance of the slice navigator background.

New in CoLocalizer Pro 5.0.3 Build 397 (May 31, 2017)

  • Improved reliability of Handoff.
  • Refreshed several graphics elements.

New in CoLocalizer Pro 5.0.2 Build 396 (May 16, 2017)

  • Fixed an issue when channel pairs in the Background Correction panel sometimes were difficult to change.
  • Added tool-tips for items in Side View Inspector.
  • Made various UI tweaks.

New in CoLocalizer Pro 5.0.1 Build 387 (Apr 24, 2017)

  • Fixed an issue when file creation and modification dates were not properly displayed in Inspector.

New in CoLocalizer Pro 5.0 Build 386 (Apr 18, 2017)

  • Full compatibility with iCloud, keeps all image files used for colocalization analysis updated on all your Macs and the forthcoming version of CoLocalizer for iPad.
  • Image files used for analysis with CoLocalizer Pro can now be saved in its own file format.
  • Now uses non-destructive editing, get back to the original image file at anytime, if needed.
  • Image files with extension .colocalizer can be opened in CoLocalizer Pro directly when sent as email attachments.
  • When changing the sequence of slices in stacks images, the numbers of original slices are now preserved.

New in CoLocalizer Pro 4.1.1 Build 365 (Mar 6, 2017)

  • Made improvements related to licensing.

New in CoLocalizer Pro 4.1.0 Build 364 (Feb 9, 2017)

  • Completely re-written functionality for viewing stacks images.
  • It is now possible to deselect all selected slices in stacks images, if needed: Edit > Deselect All.

New in CoLocalizer Pro 4.0.1 Build 358 (Feb 1, 2017)

  • Rotation and flipping of opened image is now possible if user decides to go back to the main window again after accessing Colocalization sheet and performing calculations.
  • Width of Side Inspector is now non-resizable.
  • Improved reliability of showing extensions of proprietary file formats in Side View Inspector.
  • Added fading animation when clearing selected ROI.
  • Fixed an issue when histogram for slices in stacks images in the new Side Inspector was not updated.
  • Fixed an issue when color pair channels in the Background Correction panel were displayed in the wrong sequence in some cases.

New in CoLocalizer Pro 4.0.0 Build 355 (Jan 25, 2017)

  • Significantly improved Selection tool. ROI selections are now resizable and indicate pixel dimensions of selected areas. In addition to the application menu, selection of ROI types is now possible from the toolbar menu as well.
  • Reorganized Histogram and Inspector panels by incorporating them into the main application window as parts of a new Side View Inspector.
  • Simplified and improved the appearance of Histogram.
  • Changed the name of Manual background correction option using ROI from "Selected ROI" to "Background ROI".
  • Changed Colocalization window to a sheet so that it is always confined to the main application window.
  • Pixel Information area of Colocalization sheet now shows info about Selected ROI and Image instead of Selected ROI and Scattergram ROI as it was previously.
  • Synchronized selection of color channel pairs between Background Correction panel and Colocalization sheet.
  • Simplified tooltips of coefficients in Colocalization sheet.
  • Removed the Calculate button in Colocalization sheet under the Selected ROI tab as it seemed obsolete: the values of selected ROI coefficients are now displayed instantly when the sheet pops down.
  • Removed the Calculate button in Colocalization sheet under the Scattergram tab as it seemed obsolete: the values of scattergram coefficients are now displayed instantly when the tab is opened.
  • Removed options to select Scattergram ROI and reveal selected on Scattergram pixels.
  • Colocalized pixels are now shown in the Selected ROI Preview portion of the image in Colocalization sheet instead of the main window as it was previously.
  • Removed option to export scattergram in image with Selected ROI.
  • Removed option to export image with Selected ROI.
  • Improved layout of the Export sheet.
  • Made various improvements of performance.
  • Updated ROI selection menu and Flip icons.

New in CoLocalizer Pro 3.5.7 Build 331 (Dec 22, 2016)

  • Updated View menu icons.

New in CoLocalizer Pro 3.5.6 Build 324 (Dec 13, 2016)

  • Re-organized preferences.

New in CoLocalizer Pro 3.5.5 Build 319 (Dec 7, 2016)

  • Fixed issues with licensing scheme.
  • Made enhancements of graphics.

New in CoLocalizer Pro 3.5.4 Build 316 (Dec 1, 2016)

  • Re-organized View and Tools menu items.

New in CoLocalizer Pro 3.0.0 Build 267 (Apr 30, 2015)

  • Rewritten from the ground up to leverage latest programming tools and technologies.
  • Stacks: it is now possible to open stacks of images and perform calculations on them.
  • USB digital microscopes compatibility: users can connect their Macs to the microscope camera, import images, and analyze them right in CoLocalizer.
  • Added Autosave and Versions functionality.
  • 64-bit support to take full advantage of the power and speed of modern Macs.
  • Background correction window is transformed into a panel, which shows histogram of the image according to the selected pair of channels before and after background correction.
  • Rotation and flipping are now faster and more reliable.
  • Inspector panel shows more information about images.
  • Improved self-update functionality.
  • Made numerous UI improvements and fixes.
  • Redesigned graphics to comply with Mac OS X 10.10 Yosemite aesthetics.
  • A4 is now the default paper size for PDF report.
  • Image ROI no longer disappears when rotating images.
  • Fixed an issue when modifications of images may have resulted in loss of their original dpi.
  • Fixed an issue with erroneous dpi reporting for some images.
  • Fixed an issue when clicking on the scattergram but not selecting its ROI showed pixel count.
  • Extensively revised and updated contents of the built-in Help.

New in CoLocalizer Pro 2.7.2 Build 217 (Feb 4, 2014)

  • Added Gatekeeper support.
  • Added support for Retina graphics.
  • When the app is in the full screen view, the Escape key returns it to the standard view.
  • When splitting images according to the channels, split images are now saved to a folder.
  • Improved layout and graphics of the colocalization window.
  • ROI on the scattergram under the Image ROI tab of the colocalization window is no longer selectable.
  • When saving modified, split, and exported images, BMP and PICT file formats are no longer supported.
  • Fixed an issue when text field of the Finder window lacked the names of opened files when saving modified images before quitting application.
  • Updated contents of the built-in Help.

New in CoLocalizer Pro 2.7.1 Build 214 (Feb 26, 2013)

  • When correcting background in the Manual mode using selected ROI, clicking Done now not only closes the Background Correction window, but also clears the selected ROI.
  • Now uses a crosshair cursor over the histogram.
  • Changed the way to display nil values in the Show Info panel.
  • Fixed an issue of Level and Count values for All channels in the histogram not displaying.
  • Fixed a bug when image resolution and depth were shown incorrectly for some images.
  • Fixed handling of the invalid file format - inspector was updated for the new invalid file, though file was not displayed.
  • Fixed an update of the Show Info panel and UI reset when image comes from the Merge tool (or pasted from the pasterboard).
  • Fixed a cursor update.
  • Fixed weird bugs when dragging files over main image vied, but merge panel dropped targets.
  • Fixed a refresh bug in the Show Info panel after file has been saved.
  • Fixed a refresh bug in the Show Info panel on image loading.
  • Fixed a bug when cursor was not updated with the histogram.

New in CoLocalizer Pro 2.6 (Aug 12, 2010)

  • Improved navigation of the built-in Help.
  • Revised and updated application icon.
  • Updated contents of the built-in Help and User Guide.
  • Fixed minor issues related to documentation update.

New in CoLocalizer Pro 2.5.2 (Dec 16, 2009)

  • Fixed a Mac OS X (Snow Leopard) related issue when selected image ROI and scatter gram were missing in exported images.
  • Fixed encoding of HTML report.
  • No longer creates too many pages in export when there is no selection in the image.
  • Now silently ignores unsupported file types instead of crashing.
  • Updated License Agreement for trial licenses.

New in CoLocalizer Pro 2.5.1 (Jun 28, 2009)

  • Fixed a crash whem merging some images that were not square.
  • Fixed a rare case of crash when exporting analyzed images with colocalized/selected pixels.

New in CoLocalizer Pro 2.5 (Dec 3, 2008)

  • Added more viewing options: rotate left, rotate right, flip horizontal, flip vertical.
  • Improved Check for Updates functionality.
  • Purchased licenses are now personalized.
  • Updated the Help and User Guides.
  • Fixed a case when some 300 dpi images were not properly merged.
  • Updated application and toolbar icons.
  • Made minor UI adjustments.
  • Updated License Agreement.

New in CoLocalizer Pro 2.0 (Jun 6, 2008)

  • Added universal binary support.
  • Added the ability to reveal colocalized and selected pixels.
  • Improved thumbnails loading in the image drawer.
  • Updated the Help and User Guides.
  • Fixed a rare case of crash when loading certain files that were not 72 dpi.
  • Fixed a rare case of crash when loading files with a specific PICT format.
  • Made minor UI adjustments.
  • Updated License Agreement.