Java tool that assesses the consistency of gating data across a panel of tubes
QTube produces a numerical as well as a graphical assessment of gate data consistency.
The algorithm operates directly on list-mode FCS data and requires no operator intervention. Though the underlying technology is extremely powerful, QTube itself couldn’t be easier to use.
In a nutshell, QTube looks at the similarity of event distributions in two or more tubes. Without you needing to direct its attention (via gating for example), QTube computes a fingerprint for each tube, and then compares each fingerprint against the aggregate.
It provides feedback as to how similar the distributions are, which ones (if any) are outliers, and by how much. It does all of this with a single click. If you like, you can easily export the result to Excel or other analysis or reporting software.